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b3t buffer  (Valiant Co Ltd)

 
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    Structured Review

    Valiant Co Ltd b3t buffer
    B3t Buffer, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1017 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b3t+buffer/Albumin+bovine+fraction+V/pmc05175433-73-23-80
    Average 97 stars, based on 1017 article reviews
    b3t buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Saline:

    Article Title: Humoral Antibody Responses to HIV Viral Proteins and to CD4 Among HIV Controllers, Rapid and Typical Progressors in an HIV-Positive Patient Cohort
    Article Snippet: Human anti-human antibodies were tested for specificity after purification by testing against antigens human 4D-CD4 (NIH AIDS Reagent Program) and SF2 gp120 (Chiron Corp., Emeryville, CA), which were plated in 96-well Immulon 2HB plate (Thermo Scientific, Rockford, IL) overnight at 200 ng/well in 0.1 M sodium carbonate, pH9.6. .. Plates were washed four times with 1 × phosphate-buffered saline (PBS) before 100 μl of human anti-human antibody, diluted to 3 ng/μl in B3T Buffer (8.8 g/liter NaCl, 7.87 g/liter Tris-HCl, 334.7 mg/liter EDTA, 20 g BSA Fraction V, 33.3 ml/liter fetal calf serum, 666 μl/liter Tween-20, and 0.02% Thimerosal, pH 7.4) was added to each well and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl B3T containing 1:2,000 goat anti-human IgG Fc-HRP (MP Biomedicals, Aurora, OH), or 1:4,000 goat anti-human IgA-HRP (Southern Biotech, Birmingham, AL) was added and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl of enzyme-linked immunosorbent assay (ELISA) developing solution: 2 mg OPD (Sigma, St Louis, MO) per 5 ml Substrate Buffer (10.24 g/liter citric acid, 14.2 g/liter dibasic sodium phosphate, pH 5.0) + 42 μl 30% H 2 O 2 were added to each well, and incubated for 5 min at room temperature before stopping the reaction with 50 μl 2 M sulfuric acid. .. A Victor 3 1420 multi-label plate reader (Perkin Elmer, Waltham, MA) read the plate at 493 nm.

    Incubation:

    Article Title: Humoral Antibody Responses to HIV Viral Proteins and to CD4 Among HIV Controllers, Rapid and Typical Progressors in an HIV-Positive Patient Cohort
    Article Snippet: Human anti-human antibodies were tested for specificity after purification by testing against antigens human 4D-CD4 (NIH AIDS Reagent Program) and SF2 gp120 (Chiron Corp., Emeryville, CA), which were plated in 96-well Immulon 2HB plate (Thermo Scientific, Rockford, IL) overnight at 200 ng/well in 0.1 M sodium carbonate, pH9.6. .. Plates were washed four times with 1 × phosphate-buffered saline (PBS) before 100 μl of human anti-human antibody, diluted to 3 ng/μl in B3T Buffer (8.8 g/liter NaCl, 7.87 g/liter Tris-HCl, 334.7 mg/liter EDTA, 20 g BSA Fraction V, 33.3 ml/liter fetal calf serum, 666 μl/liter Tween-20, and 0.02% Thimerosal, pH 7.4) was added to each well and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl B3T containing 1:2,000 goat anti-human IgG Fc-HRP (MP Biomedicals, Aurora, OH), or 1:4,000 goat anti-human IgA-HRP (Southern Biotech, Birmingham, AL) was added and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl of enzyme-linked immunosorbent assay (ELISA) developing solution: 2 mg OPD (Sigma, St Louis, MO) per 5 ml Substrate Buffer (10.24 g/liter citric acid, 14.2 g/liter dibasic sodium phosphate, pH 5.0) + 42 μl 30% H 2 O 2 were added to each well, and incubated for 5 min at room temperature before stopping the reaction with 50 μl 2 M sulfuric acid. .. A Victor 3 1420 multi-label plate reader (Perkin Elmer, Waltham, MA) read the plate at 493 nm.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Humoral Antibody Responses to HIV Viral Proteins and to CD4 Among HIV Controllers, Rapid and Typical Progressors in an HIV-Positive Patient Cohort
    Article Snippet: Human anti-human antibodies were tested for specificity after purification by testing against antigens human 4D-CD4 (NIH AIDS Reagent Program) and SF2 gp120 (Chiron Corp., Emeryville, CA), which were plated in 96-well Immulon 2HB plate (Thermo Scientific, Rockford, IL) overnight at 200 ng/well in 0.1 M sodium carbonate, pH9.6. .. Plates were washed four times with 1 × phosphate-buffered saline (PBS) before 100 μl of human anti-human antibody, diluted to 3 ng/μl in B3T Buffer (8.8 g/liter NaCl, 7.87 g/liter Tris-HCl, 334.7 mg/liter EDTA, 20 g BSA Fraction V, 33.3 ml/liter fetal calf serum, 666 μl/liter Tween-20, and 0.02% Thimerosal, pH 7.4) was added to each well and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl B3T containing 1:2,000 goat anti-human IgG Fc-HRP (MP Biomedicals, Aurora, OH), or 1:4,000 goat anti-human IgA-HRP (Southern Biotech, Birmingham, AL) was added and incubated at room temperature for 45 min. Wells were washed as before, then 100 μl of enzyme-linked immunosorbent assay (ELISA) developing solution: 2 mg OPD (Sigma, St Louis, MO) per 5 ml Substrate Buffer (10.24 g/liter citric acid, 14.2 g/liter dibasic sodium phosphate, pH 5.0) + 42 μl 30% H 2 O 2 were added to each well, and incubated for 5 min at room temperature before stopping the reaction with 50 μl 2 M sulfuric acid. .. A Victor 3 1420 multi-label plate reader (Perkin Elmer, Waltham, MA) read the plate at 493 nm.



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